Application

Research Sub CategoryNeurodegenerative DiseasesNeurofilament & Neuron MetabolismRNA Binding Protein (RBP)

Research CategoryNeuroscience

Anti-Iron Pegulatory Protein 1 Antibody detects level of Iron Pegulatory Protein 1 & has been published & validated for use in ELISA & WB.

Western blot: 1-10 µg/mL using ECL.
Immunohistochemistry: Not tested. Recommended testing antibody dilution:2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Iron regulatory proteins (IRP-1 and IRP-2) are cytoplasmic mRNA-binding proteins that control intracellular iron levels by regulating the translation of ferritin, Tfrs, and other proteins congaing iron-responsive element (IRE) located at the 5′-UTR. IRP binds to IREs with high affinity in situation of iron starvation. Binding of IRP to IRE of ferritin represses its transcription. However, when cells are iron replete, IRPs lose their capacity to bind IREs, allowing efficient translation of ferritin and reducing Tfr mRNA half-life. Although IRP1 and IRP-2 share significant protein sequence homology, they differ in tissue distribution and mode of regulation. IRP-2/IRE-BP2 (rat/human 963 aa) is relatively less abundant than IRP-1 and lacks aconitase activity. It has a unique 73-aa insertion in the N-terminus. In the presence of high iron levels, IRP-2 is rapidly targeted to proteasome-mediated degradation. Although, both IRPs bind the consensus IREs, it is also shown that IRP-2 can recognize an exclusive IRE subset.

Immunogen

Synthetic peptide near the N-terminus of rat IRP-1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

ImmunoAffinity Purified

Format: Purified

Liquid in PBS with 0.1% BSA.

Specificity

Other species have not yet been tested. The immunogen sequence is 94% conserved in mouse, and 89% in human and rabbit IRP-1.

Iron regulatory protein 1 (IRP-1). The immunogen sequence has no significant homology with IRP-2.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.